Asian Journal of Bio Science

A. S. Gawande ¹, Dipali V. Ghive ¹, R. B. Ghorade ¹, Neeta P. Barbade ¹, Swati R. Pote ¹

Affiliations
1 Dept. of Botany, Tissue Culture Lab, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN

Abstract

For callus induction studies explants i.e., shoot tips hypocotyls and ischolar_mains were excised from six days aseptically grown seedlings. NAA 2 mg/l+BAP 2 mg/l was found best among various combinations used for induction of callus. For differentiation of callus BAP 1 mg/l+NAA 0.5 mg/l treatment combination was found to be the best that resulted in maximum number of shoot lets, for differentiation followed by profuse ischolar_maining to the regenerated shoots. For the completely developed plantlets, the maximum survival was observed with the hardening treatment where in the plants were directly transferred into polybags were kept in mist chamber.

Keywords

Callus, Chickpea, BAP, NAA.

Full Text

     

Dipali V. Ghive ¹, Nita P. Barabde ¹, Swati R. Pote ¹

Affiliations
1 Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN

Abstract

For most seed technologists and commercial seedmen, viability means that a seed is capable of germinating and producing normal seedlings. Viability denotes the degree to which a seed is alive, metabolically active and posses enzymes capable of catalyzing metabolic reactions needed for germination and seedling growth. This meaning deals with tissue viability as well as viability of the entire seed. Seed viability is probably highest at the time of physiological maturity though environmental conditions on the apparent plant may not permit germination. After physiological maturity, the viability of seed gradually declines.

Full Text